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Research Article |

Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19

Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validate an HPLC method for the dosage of AZT and the search for its degradation products. The validated method was carried out on an octadecylsylanized column (250 mm × 4.6 mm, 5 μm) using a mobile phase which consisted of a methanol/phosphate buffer mixture at pH 7.5 (80/20 v/v). The flow rate was set at 1.5 ml/min for a column temperature of 50°C and the UV detection wavelength at 210 nm. Forced degradation studies were carried out under the conditions: acidic, alkaline, oxidizing, UV light and thermal, in order to determine the degradation profiles. The validation parameters which are the linearity domain, the limits of detection and quantification; repeatability and accuracy were satisfactory. All samples showed consistent azithromycin contents. However, related substances and degradation products have been found in certain specialties. The validation of the HPLC method gave reliable results which allowed the determination of AZT and the search for degradation products and impurities.

COVID-19, Azithromycin, Validation, HPLC, Degradation Products

APA Style

Yao, M. A., Kpaibe, A. S., Yao, T. J. S., Able, N., Bony, N., et al. (2024). Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Science Journal of Analytical Chemistry, 12(1), 1-6. https://doi.org/10.11648/j.sjac.20241101.11

ACS Style

Yao, M. A.; Kpaibe, A. S.; Yao, T. J. S.; Able, N.; Bony, N., et al. Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Sci. J. Anal. Chem. 2024, 12(1), 1-6. doi: 10.11648/j.sjac.20241101.11

AMA Style

Yao MA, Kpaibe AS, Yao TJS, Able N, Bony N, et al. Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Sci J Anal Chem. 2024;12(1):1-6. doi: 10.11648/j.sjac.20241101.11

Copyright © 2024 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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