Volume 5, Issue 1, January 2017, Page: 1-7
Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine
Sharifah Ghaythan Dbsan Alharthi, Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia; Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
Ayman Abdelaziz Osman, Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia; Departmen of Chemistry, College of Science and Education, University of West Kurdofan, EL Nuhud, Sudan
Salma Ali Altamimi, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
Fatma Elzahraa Ali, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
Received: Dec. 31, 2016;       Accepted: Jan. 12, 2017;       Published: Feb. 6, 2017
DOI: 10.11648/j.sjac.20170501.11      View  3325      Downloads  101
Abstract
A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.
Keywords
Gemifloxacin Mesylate, Spectrofluorometry, Terbium(III), Hexamine, Ternary Complex
To cite this article
Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali, Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine, Science Journal of Analytical Chemistry. Vol. 5, No. 1, 2017, pp. 1-7. doi: 10.11648/j.sjac.20170501.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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