Volume 5, Issue 1, January 2017, Page: 8-11
Near-Infrared Reflectance Spectroscopy (NIRs) for Determination of Tryptophan Content in Quality Protein Maize (QPM)
Legesse Shiferaw, Ethiopian Institute of Agricultural Research, Agricultural Quality Research Laboratory, Adiss Abeba, Ethiopia
Girmay Tsegay, Ethiopian Institute of Agricultural Research, Agricultural Quality Research Laboratory, Adiss Abeba, Ethiopia
Gelila Asamenew, Ethiopian Institute of Agricultural Research, Agricultural Quality Research Laboratory, Adiss Abeba, Ethiopia
Received: Dec. 25, 2016;       Accepted: Jan. 5, 2017;       Published: Feb. 10, 2017
DOI: 10.11648/j.sjac.20170501.12      View  3876      Downloads  113
Abstract
Quality protein maize (QPM) has approximately twice tryptophan (Trp) and lysine (Lys) concentrations in protein compared to normal maize. Because several genetic systems control the protein quality of QPM, it is essential to regularly monitor Trp and/or Lys in QPM breeding programs through laboratory analysis. The objective of the study was to evaluate the capability of Near –Infrared Reflectance Spectroscopy (NIRS) method in determining tryptophan content of QPM which enhance the efficiency of QPM research efforts by partially replacing more expensive and time-consuming wet chemistry analysis. 268 maize samples were used to develop NIRS models for Tryptophan content. Standard error (SEC) and coefficient of determination for the calibration were 0.007 and 0.76 respectively. When the NIRS model was subjected to external validation with 40 S2 lines from QPM breeding populations, the standard error of prediction (SEP) for validation and coefficient of determination between NIRS and the chemical data were 0.008 and 0.84 respectively. Therefore, from the result it is confirmed that NIRS model is effective tool for screening of QPM from normal maize.
Keywords
Quality Protein Maize, Tryptophan, Lysine, NIRS
To cite this article
Legesse Shiferaw, Girmay Tsegay, Gelila Asamenew, Near-Infrared Reflectance Spectroscopy (NIRs) for Determination of Tryptophan Content in Quality Protein Maize (QPM), Science Journal of Analytical Chemistry. Vol. 5, No. 1, 2017, pp. 8-11. doi: 10.11648/j.sjac.20170501.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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