Volume 8, Issue 1, March 2020, Page: 12-17
LC/MS Analysis of Cyclo Fatty Acid-containing Triacylglycerols in Cottonseed Oil
Shuji Hirayama, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
Daichi Shinozaki, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
Yoshiya Izumi, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
Received: Dec. 22, 2019;       Accepted: Jan. 15, 2020;       Published: Jan. 31, 2020
DOI: 10.11648/j.sjac.20200801.13      View  408      Downloads  185
We measured the constituent triacylglycerol of cotton oil, the only edible oil containing a cyclo fatty acid. Due to the difficulty of obtaining a standard for triacylglycerols containing cyclo fatty acids, cotton oil is analyzed using cyclo triacylglycerol in kapok oil as a reference. Analytical methods used liquid chromatography fast atom bombardment mass spectrometry (LC / FAB-MS) using a matrix of 3% m-NBA containing 3 mM NaCl, and orthogonal transfer method for APCI / TOF-MS. FAB mass spectral properties of triacylglycerols containing cyclo fatty acids can be observed with [M + Na]+, [M-1]+ molecular ions, and [M- (RCOO)] + fragments. In the orthogonal APCI / TOF-MS spectrum, only the [M-1] + molecular ion appears, so that the presence of triacylglycerol containing CPFA becomes apparent. On the other hand, chain triacylglycerol can be observed as [M + 1]+ molecular ions and [M- (RCOO)]+ fragments. Based on these observations, triacylglycerols containing ten types of cyclo fatty acids were successfully successfully detected in crude cottonseed oil. In addition, the APCI/TOF-MS technique successfully detected dilinoleoyl-marvalyl-glycerol and palmtoyl-linoleoyl-marvalyl glycerol in the purified cottonseed oil.
LC/FAB-MS, Cyclo Fatty Acid, Kapok Oil, Cottonseed Oil
To cite this article
Shuji Hirayama, Daichi Shinozaki, Yoshiya Izumi, LC/MS Analysis of Cyclo Fatty Acid-containing Triacylglycerols in Cottonseed Oil, Science Journal of Analytical Chemistry. Vol. 8, No. 1, 2020, pp. 12-17. doi: 10.11648/j.sjac.20200801.13
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