Volume 5, Issue 6, November 2017, Page: 90-97
Quantitation of Aflatoxins in Food Materials Using HPLC-FLD Method
Fevzi Kilicel, Faculty of Science, Department of Chemistry, Karamanoglu Mehmetbey University, Karaman, Turkey
Hacer Sibel Karapinar, Faculty of Science, Department of Chemistry, Karamanoglu Mehmetbey University, Karaman, Turkey
Aysel Cimen, Faculty of Science, Department of Chemistry, Karamanoglu Mehmetbey University, Karaman, Turkey
Received: Apr. 4, 2017;       Accepted: Apr. 22, 2017;       Published: Nov. 14, 2017
DOI: 10.11648/j.sjac.20170506.11      View  230      Downloads  15
Abstract
In this study, a sensitive high performance liquid chromatography coupled to a fluorescence detector (HPLC-FLD) method after post-column derivatisation was applied for the presence of aflatoxins (AFs) in 45 samples (dried apricots, raisins, dried figs, nuts, peanuts, almonds, corn, redpepper, blackpepper, bread and moldy cheese). Samples were collected four seasons from several open market places in Karaman/Turkey and checked for AFs levels. At the same time bread samples were incubated for seven day outdoors. The purpose of this experiment is to determine the time required to reach the critical aflatoxin amounts in the bread that we consumed in our home. AFs were present in 75% of dried apricots (6/8), 100% of raisins (8/8), 100% of dried figs (8/8), 75% of nuts (6/8), 50% of peanuts (4/8), 50% of almonds (4/8), 100% of corn (8/8), 100% of blackpepper (8/8), 100% of redpepper (8/8), 78.5% of bread (11/14), 75% of moldy cheese (3/4) at total AF levels ranging. Eight redpepper samples were above the European maximum tolerable limit (MTL) of 8 µg kg-1 for AFB1, while total AFs concentration exceeded the MTL of 10 µg kg-1 in eight redpepper samples. The amount of aflatoxin in other samples is lower than the value of European MLT.
Keywords
Aflatoxins, HPLC-FLD, Food Material, Derivatization, Turkey
To cite this article
Fevzi Kilicel, Hacer Sibel Karapinar, Aysel Cimen, Quantitation of Aflatoxins in Food Materials Using HPLC-FLD Method, Science Journal of Analytical Chemistry. Vol. 5, No. 6, 2017, pp. 90-97. doi: 10.11648/j.sjac.20170506.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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