An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals
Zuhair Abdul Al-Ameer Khammas,
Noora Saad Mubdir
Issue:
Volume 2, Issue 5, September 2014
Pages:
47-54
Received:
13 September 2014
Accepted:
24 September 2014
Published:
30 September 2014
Abstract: A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.
Abstract: A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point ...
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Kinetic Studies on the Recovery of Pb2+and UO22+ from Aqueous Solutions into Chloroform Solution of N,N’-Ethylenebis(4-Butanoyl-2,4-Dihydro-5-Methyl-2-Phenyl-3H-Pyrazol-3-Oneimine) Schiff Base
Chukwu Uche John,
Godwin Jackson,
Onuigbo Somadina Benson
Issue:
Volume 2, Issue 5, September 2014
Pages:
55-58
Received:
28 September 2014
Accepted:
13 October 2014
Published:
20 October 2014
Abstract: The kinetic studies on the recovery of UO22+ and Pb2+ from aqueous solutions into chloroform solution of N,N’-ethylenebis(4-butanoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-oneimine) schiff base H2BuEtP has been investigated and the rate of reaction found to be first order with respect to both metal ions (UO22+ and Pb2+). The rate of extraction was equally dependent on the inverse of hydrogen ion concentration for both metal ions. The rate constant Kf was equally calculated from intercepts of plots to be 0.491min-1 for UO22+ and 0.411 min-1 Pb2+ metal ions. Optimum percentage extraction of 99.714% was observed for Pb2+ metal ion at 30mins equilibration time and 98.216% for UO22+ metal ion at 20mins equilibration time. The study on mechanism of extraction for the metal ions from the aqueous phase into the organic phase showed that the formation of the first chelate ring of mono-pyrazolonato metal complex is the rate determining step which involved a slow bond-weaking effect by the bidendate H2BuEtP on the water molecule.
Abstract: The kinetic studies on the recovery of UO22+ and Pb2+ from aqueous solutions into chloroform solution of N,N’-ethylenebis(4-butanoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-oneimine) schiff base H2BuEtP has been investigated and the rate of reaction found to be first order with respect to both metal ions (UO22+ and Pb2+). The rate of extraction ...
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