Determination of Quinine Clearance in Chronic Renal Failure During Haemodialysis
Christophe N’cho Amin,
Philippe André Sawa Kpaibé,
Nicaise François Bony,
Gildas Komenan Gbassi,
Michèle Aké,
Apollinaire Gnionsahé,
Eugène Atindehou
Issue:
Volume 4, Issue 3, May 2016
Pages:
26-29
Received:
6 April 2016
Accepted:
18 April 2016
Published:
3 May 2016
Abstract: Hemodialysis is a method that used to achieve the extracorporeal removal of waste products such as creatinine and urea and free water from the blood when the kidneys are in a state of renal failure. However, this treatment may reduce the effectiveness of certain drugs. It is necessary to study the dialysance of quinine, a drug used in first intention for the treatment of severe malaria due to the fragility of uremic patients and the presence of malaria in endemic areas. This study has been conducted at the hemodialysis center of SAMU (Service d’Aide Médicale d’Urgence) in Abidjan Cocody. Twenty one (21) subjects with chronic renal failure aged of 24 to 50 years were enrolled. Two groups of subjects were formed. Each patient of the first group (9 subjects) received per person one tablet of 500 mg of quinine base (single dose) before the hemodialysis started. The second group (12 subjects) received in perfusion 5% glucose solution (250 mL) containing 10 mg.kg-1 of quinine base during 4 hours. The perfusion system and the hemodialysis system were conducted simultaneously. The concentrations of quinine in the tablet, the perfusion solution and the human blood were determined by a validated high performance liquid chromatography method. Quinine content in tablet and perfusion solution was in agreement with the manufacturer's specifications. The clearance of quinine was 23.67 mL.min-1. It appeared of this study that quinine fraction extracted did not require dose adjustment.
Abstract: Hemodialysis is a method that used to achieve the extracorporeal removal of waste products such as creatinine and urea and free water from the blood when the kidneys are in a state of renal failure. However, this treatment may reduce the effectiveness of certain drugs. It is necessary to study the dialysance of quinine, a drug used in first intenti...
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Cloud Point Extraction of Carbendazim Pesticide in Foods and Environmental Matrices Prior to Visible Spectrophotometric Determination
Zuhair A-A Khammas,
Suher Salah Ahmad
Issue:
Volume 4, Issue 3, May 2016
Pages:
30-41
Received:
13 April 2016
Accepted:
22 April 2016
Published:
23 May 2016
Abstract: Two simple eco-friendly methods are described for nano-determination of carbendazim (MBC) pesticide in real samples. These methods are based on oxidation of MBC pesticide with Fe (III) ions in acidic medium. The formed Fe(II) ions reacts with potassium ferricyanide to form blue colored product (method A) which can easily be extracted into nonionic surfactant solution of Triton X-114 at cloud point temperature (CPT) of 55°C and MBC determined spectrophotometrically at absorption maximum of 685 nm with apparent molar absorptivity of 2.07x104 L mol-1 cm-1. The Method B is based on the reaction of the formed Fe (II) with 2, 2’-bipyridyl to form a stable orange colored complex which can also be extracted by Triton X-114 at the same CPT and MBC determined spectrophotometrically at absorption maximum of 521 nm with apparent molar absorptivity of 1.83x104 L mol-1 cm-1. Optimization of the experimental parameters was described and interferences study also examined. Under the optimum conditions established, the calibration graphs for MBC were linear in the range of 0.5-13 and 1-20 ng mL-1, giving the detection limits of 0.46 and 0.49 ng mL-1 with enrichment factors of 85.7 and 38.9 fold for method A and B respectively. The average percent recoveries in the real spiked samples were (97.86±1.06%) and (98.66±0.93%), giving a precision in terms of %RSD in the range of 1.25-2.97% and 0.37-1.42% for method A and B respectively. The proposed methods were applied to the determination of MBC in vegetables, orange, and water samples.
Abstract: Two simple eco-friendly methods are described for nano-determination of carbendazim (MBC) pesticide in real samples. These methods are based on oxidation of MBC pesticide with Fe (III) ions in acidic medium. The formed Fe(II) ions reacts with potassium ferricyanide to form blue colored product (method A) which can easily be extracted into nonionic ...
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