Validated Analytical Method Development of Inosine Pranobex in Drug Products by Thin Layer Chromatography
Issue:
Volume 2, Issue 6, November 2014
Pages:
59-66
Received:
8 October 2014
Accepted:
28 October 2014
Published:
10 November 2014
DOI:
10.11648/j.sjac.20140206.11
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Abstract: Thin layer chromatography (TLC) with densitometry has been established for the identification and the quantification of inosine pranobex in drug substance and drug products. Inosine pranobex is a combination of inosine, acetamidobenzoic acid, and dimethylaminoisopropanol. UV densitometry was performed in absorbance mode at 260 nm. The separation was carried out on aluminum sheet of silica gel 60 f 254 [chloroform - methanol- - toluene -10% ammonia solution (6:5:1: 0.1% v/v)] as mobile phase. Linearity range was found to be 1-12, 2-12, 2-20 and 2-16 µg/ml for inosine pranobex, inosine, acetamidobenzoic acid, and dimethylaminoisopropanol with the mean percentage recoveries 99.74± 1.73%, 99.88 ± 1.75%, 99.56 ±1.08%, and 99.36 ± 0.71% respectively, (Correlation coefficient r2 = 0.9998 for inosine pranobex, r2 = 0.09999 for inosine, r2 = 0.9998 for acetamidobenzoic acid and r2= 0.9998 for dimethylaminoisopropanol). The detection and quantification limits for inosine pranobex and other components are also reported. The presented method was validated according to ICH guidelines. Statistical comparison of the results was performed using Student's t-test and F-ratio at 95% confidence level, and there was no significant difference between the reference and proposed method with regard to accuracy and precision. It could be said that the validated TLC-densitometry method is suitable for the routine analysis of inosine pranobex in quantity control laboratories.
Abstract: Thin layer chromatography (TLC) with densitometry has been established for the identification and the quantification of inosine pranobex in drug substance and drug products. Inosine pranobex is a combination of inosine, acetamidobenzoic acid, and dimethylaminoisopropanol. UV densitometry was performed in absorbance mode at 260 nm. The separation wa...
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Adsorption Studies for the Removal Heavy Metal by Chitosan-G-Poly (Acrylicacid-Co-Acrylamide) Composite
Shyam R. Khairkar,
Arun R. Raut
Issue:
Volume 2, Issue 6, November 2014
Pages:
67-70
Received:
24 September 2014
Accepted:
25 October 2014
Published:
19 December 2014
DOI:
10.11648/j.sjac.20140206.12
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Abstract: In present research work natural bio polymer “Chitosan” was grafted onto Acrylic acid and Acrylamide and adsorption of some heavy metal studied by batch method. Synthesis of grafted chitosan supersorbent was synthesized by radical polymerization method. The adsorption of heavy metal cations, Cu(II), Cd(II), Ni(II), and Pb(II) from aqueous solution by newly-synthesized superabsorbent composite of chitosan-g-poly (acrylicacid-co-acrylamide) was investigated. The superabsorbent gel was characterized on the basis of FTIR, X-ray differation, Scanning electron Microscopy and thermal properties. The percentage adsorptions of metal ions on adsorbent were determined by batch methods using atomic absorption spectrophotometry (AAS). The effect of experimental parameters, such as pH, treatment time, temperature, adsorbent dose, initial metal ion concentra¬tion on the removal of metal ions was also studied. Chitosan-g-poly (acrylicacid-co-acrylamide) proved to be an effective adsorbent for the removal of different heavy metal ions from aqueous solution.
Abstract: In present research work natural bio polymer “Chitosan” was grafted onto Acrylic acid and Acrylamide and adsorption of some heavy metal studied by batch method. Synthesis of grafted chitosan supersorbent was synthesized by radical polymerization method. The adsorption of heavy metal cations, Cu(II), Cd(II), Ni(II), and Pb(II) from aqueous solution ...
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